Table 2 Known miRNAs identified in the four life stages libraries of An. funestus

afu-bantam

UGAGAUCACUUUGAAAGCUGAU

22

KB669192.1_supercont1.62:255022..255087

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-let-7

UGAGGUAGUUGGUUGUAUAGU

21

KB669047.1_supercont1.49:698595..698654

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-1

UGGAAUGUAAAGAAGUAUGGAG

22

KB668556.1_supercont1.130:34984..35060

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

afu-miR-10

ACCCUGUAGAUCCGAAUUUGUU

22

KB668870.1_supercont1.33:182453..182515

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-100

AACCCGUAGAUCCGAACUUGUG

22

KB669047.1_supercont1.49:703012..703075

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-1000

AUAUUGUCCUGUCACAGCAGUA

22

KB669169.1_supercont1.6:205456..205544

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-11

CAAGAACUUGGUACUGUGACCUGUG

25

KB669169.1_supercont1.6:410485..410557

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-1175

AAGUGGAGUAGUGGUCUCAUCGCU

24

KB669358.1_supercont1.77:114656..114718

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-124

UAAGGCACGCGGUGAAUGCCA

21

KB668556.1_supercont1.130:267597..267658

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-125

UCCCUGAGACCCUAACUUGUGAC

23

KB669047.1_supercont1.49:697981..698042

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-133

UUGGUCCCCUUCAACCAGCUGU

22

KB668872.1_supercont1.331:18310..18396

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-137

UAUUGCUUGAGAAUACACGUAG

22

KB669203.1_supercont1.63:461201..461262

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-13b

UAUCACAGCCAUUUUGACGA

20

KB668788.1_supercont1.256:85269..85330

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-14

UCAGUCUUUUUCUCUCUCCUAU

22

KB668222.1_supercont1.10:1664927..1664991

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-184

UGGACGGAGAACUGAUAAGGGC

22

KB668984.1_supercont1.432:34990..35049

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-1890

UGAAAUCUUUGAUUAGGUCUGG

22

KB668768.1_supercont1.238:330029..330096

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-1891

UGAGGAGUUAAUUUGCGUGUUU

22

KB668738.1_supercont1.210:313240..313298

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-190

AGAUAUGUUUGAUAUUCUUGGUUG

24

KB668683.1_supercont1.161:159767..159832

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-2-1/2

UAUCACAGCCAGCUUUGAUGAGC

23

KB668788.1_supercont1.256:84051..84114

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-210

CUUGUGCGUGUGACAACGGCUAU

23

KB668844.1_supercont1.306:43398..43456

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-219

AGAGUUGUGACUGGACAUCCGUG

23

KB668767.1_supercont1.237:131113..131179

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-263a

AAUGGCACUGGAAGAAUUCACGGG

24

KB668222.1_supercont1.10:692930..692993

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-263b

CUUGGCACUGGGAGAAUUCACAG

23

KB668812.1_supercont1.278:190692..190757

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-275

UCAGGUACCUGAAGUAGCGCGCG

23

KB668400.1_supercont1.116:398864..398930

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-276

UAGGAACUUCAUACCGUGCUCU

22

KB668722.1_supercont1.197:198964..199032

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-277

UAAAUGCACUAUCUGGUACGACA

23

KB669169.1_supercont1.6:1092927..1092992

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-278

ACGGACGAUAGUCUUCAACGACC

23

KB668223.1_supercont1.100:30655..30714

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-279

UGACUAGAUCCACACUCAUUAA

22

KB668289.1_supercont1.106:353827..353890

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-281

AAGAGAGCUAUCCGUCGACAGU

22

KB669503.1_supercont1.90:359505..359565

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-282

UAGCCUCUUCUAGGCUUUGUCU

22

KB669503.1_supercont1.90:578663..578726

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-283

AAAUAUCAGCUGGUAAUUCUAGG

23

KB668836.1_supercont1.3:514580..514646

\(\surd\) \(\surd\) \(\times\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\times\)

\(\surd\) \(\surd\) \(\times\)

afu-miR-286

UGACUAGACCGAACACUCGCGUCCU

25

KB668445.1_supercont1.120:344766..344839

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-305

AUUGUACUUCAUCAGGUGCUCUGG

24

KB668400.1_supercont1.116:390085..390147

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-307

UCACAACCUCCUUGAGUGAGCGA

23

KB668223.1_supercont1.100:429991..430050

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

afu-miR-308

CGCAGUAUAUUCUUGUGAACUUG

23

KB668933.1_supercont1.387:20015..20073

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-309

UCACUGGGCAAAGUUUGUCGCA

22

KB668445.1_supercont1.120:344139..344203

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

afu-miR-315

UUUUGAUUGUUGCUCAGAAAGCC

23

KB668747.1_supercont1.219:307337..307401

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-317

UGAACACAUCUGGUGGUAUCUCAGU

25

KB669169.1_supercont1.6:1109058..1109126

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-34

UGGCAGUGUGGUUAGCUGGUUGU

23

KB669169.1_supercont1.6:1091079..1091145

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-375

UUUGUUCGUUUGGCUCGAGUUA

22

KB669281.1_supercont1.70:314839..314901

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-7

UGGAAGACUAGUGAUUUUGUUGUU

24

KB668798.1_supercont1.265:312209..312271

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-79

AUAAAGCUAGAUUACCAAAGCAU

23

KB668930.1_supercont1.384:9585..9649

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-8

UAAUACUGUCAGGUAAAGAUGUC

23

KB668666.1_supercont1.146:27512..27573

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-87

CCAGCCUGAAAUUUGCUAAACCU

23

KB669058.1_supercont1.5:469321..469384

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-927-5p

UUUAGAAUUCCUACGCUUUACC

22

KB668660.1_supercont1.140:64296..64359

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-929

AAAUUGACUCUAGUAGGGAGU

21

KB668836.1_supercont1.3:1318319..1318378

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-92a

UAUUGCACUUGUCCCGGCCUAU

22

KB668836.1_supercont1.3:1691007..1691065

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-92b

AAUUGCACUUGUCCCGGCCUGC

22

KB668836.1_supercont1.3:1704691..1704754

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-957

UGAAACCGUCCAAAACUGAGGC

22

KB669514.1_supercont1.91:212823..212889

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-970

UCAUAAGACACACGCGGCUAU

21

KB668797.1_supercont1.264:230410..230478

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-981

UUCGUUGUCGACGAAACCUGCA

22

KB668389.1_supercont1.115:58097..58172

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\surd\) \(\times\)

afu-miR-988

CCCCUUGUUGCAAACCUCACGC

22

KB669391.1_supercont1.8:63805..63867

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

afu-miR-993

GAAGCUCGUUUCUAUAGAGGUAUC

24

KB668870.1_supercont1.33:220731..220822

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-996

UGACUAGAUUACAUGCUCGUCU

22

KB668289.1_supercont1.106:354280..354344

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-9a

UCUUUGGUUAUCUAGCUGUAUGA

23

KB668816.1_supercont1.281:204094..204152

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-mir-9b

ACUUUGGUGAUUUAGCUGUAUGU

23

KB668930.1_supercont1.384:9150..9215

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

afu-miR-9c

UCUUUGGUAUUCUAGCUGUAGA

22

KB668930.1_supercont1.384:12370..12438

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

afu-miR-iab-4

ACGUAUACUGAAUGUAUCCUGA

22

KB668694.1_supercont1.171:357100..357157

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-12

UGAGUAUUACAUCAGGUACUGGU

23

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-306

UCAGGUACUGGAUGACUCUCAG

22

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-mir-965

UAAGCGUAUAGCUUUUCCCAUU

22

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-989

UGUGAUGUGACGUAGUGGUAC

21

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-1174

UCAGAUCUACUUCAUACCCAUG

22

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

afu-miR-1889

ACACAUUACAGAUUGGGAUUA

21

Unknown

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)