Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing

Allam, Mushal; Spillings, Belinda L.; Abdalla, Hiba; Mapiye, Darlington; Koekemoer, Lizette L.; Christoffels, Alan

doi:10.1186/s12936-016-1591-0

Table 2 Known miRNAs identified in the four life stages libraries of An. funestus

From: Identification and characterization of microRNAs expressed in the African malaria vector Anopheles funestus life stages using high throughput sequencing

miRNA name	Sequences	Length	Location	Egg	Larva	Pupa	Unfed adult females
afu-bantam	UGAGAUCACUUUGAAAGCUGAU	22	KB669192.1_supercont1.62:255022..255087	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-let-7	UGAGGUAGUUGGUUGUAUAGU	21	KB669047.1_supercont1.49:698595..698654	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-1	UGGAAUGUAAAGAAGUAUGGAG	22	KB668556.1_supercont1.130:34984..35060	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\times\)
afu-miR-10	ACCCUGUAGAUCCGAAUUUGUU	22	KB668870.1_supercont1.33:182453..182515	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-100	AACCCGUAGAUCCGAACUUGUG	22	KB669047.1_supercont1.49:703012..703075	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-1000	AUAUUGUCCUGUCACAGCAGUA	22	KB669169.1_supercont1.6:205456..205544	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-11	CAAGAACUUGGUACUGUGACCUGUG	25	KB669169.1_supercont1.6:410485..410557	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-1175	AAGUGGAGUAGUGGUCUCAUCGCU	24	KB669358.1_supercont1.77:114656..114718	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-124	UAAGGCACGCGGUGAAUGCCA	21	KB668556.1_supercont1.130:267597..267658	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-125	UCCCUGAGACCCUAACUUGUGAC	23	KB669047.1_supercont1.49:697981..698042	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-133	UUGGUCCCCUUCAACCAGCUGU	22	KB668872.1_supercont1.331:18310..18396	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-137	UAUUGCUUGAGAAUACACGUAG	22	KB669203.1_supercont1.63:461201..461262	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-13b	UAUCACAGCCAUUUUGACGA	20	KB668788.1_supercont1.256:85269..85330	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-14	UCAGUCUUUUUCUCUCUCCUAU	22	KB668222.1_supercont1.10:1664927..1664991	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-184	UGGACGGAGAACUGAUAAGGGC	22	KB668984.1_supercont1.432:34990..35049	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-1890	UGAAAUCUUUGAUUAGGUCUGG	22	KB668768.1_supercont1.238:330029..330096	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-1891	UGAGGAGUUAAUUUGCGUGUUU	22	KB668738.1_supercont1.210:313240..313298	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-190	AGAUAUGUUUGAUAUUCUUGGUUG	24	KB668683.1_supercont1.161:159767..159832	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-2-1/2	UAUCACAGCCAGCUUUGAUGAGC	23	KB668788.1_supercont1.256:84051..84114	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-210	CUUGUGCGUGUGACAACGGCUAU	23	KB668844.1_supercont1.306:43398..43456	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-219	AGAGUUGUGACUGGACAUCCGUG	23	KB668767.1_supercont1.237:131113..131179	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-263a	AAUGGCACUGGAAGAAUUCACGGG	24	KB668222.1_supercont1.10:692930..692993	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-263b	CUUGGCACUGGGAGAAUUCACAG	23	KB668812.1_supercont1.278:190692..190757	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-275	UCAGGUACCUGAAGUAGCGCGCG	23	KB668400.1_supercont1.116:398864..398930	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-276	UAGGAACUUCAUACCGUGCUCU	22	KB668722.1_supercont1.197:198964..199032	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-277	UAAAUGCACUAUCUGGUACGACA	23	KB669169.1_supercont1.6:1092927..1092992	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-278	ACGGACGAUAGUCUUCAACGACC	23	KB668223.1_supercont1.100:30655..30714	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-279	UGACUAGAUCCACACUCAUUAA	22	KB668289.1_supercont1.106:353827..353890	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-281	AAGAGAGCUAUCCGUCGACAGU	22	KB669503.1_supercont1.90:359505..359565	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-282	UAGCCUCUUCUAGGCUUUGUCU	22	KB669503.1_supercont1.90:578663..578726	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-283	AAAUAUCAGCUGGUAAUUCUAGG	23	KB668836.1_supercont1.3:514580..514646	\(\surd\) \(\surd\) \(\times\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\times\)	\(\surd\) \(\surd\) \(\times\)
afu-miR-286	UGACUAGACCGAACACUCGCGUCCU	25	KB668445.1_supercont1.120:344766..344839	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-305	AUUGUACUUCAUCAGGUGCUCUGG	24	KB668400.1_supercont1.116:390085..390147	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-307	UCACAACCUCCUUGAGUGAGCGA	23	KB668223.1_supercont1.100:429991..430050	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\times\)
afu-miR-308	CGCAGUAUAUUCUUGUGAACUUG	23	KB668933.1_supercont1.387:20015..20073	\(\surd\) \(\times\) \(\surd\)	\(\times\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-309	UCACUGGGCAAAGUUUGUCGCA	22	KB668445.1_supercont1.120:344139..344203	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\times\)	\(\times\) \(\times\) \(\times\)	\(\times\) \(\times\) \(\times\)
afu-miR-315	UUUUGAUUGUUGCUCAGAAAGCC	23	KB668747.1_supercont1.219:307337..307401	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-317	UGAACACAUCUGGUGGUAUCUCAGU	25	KB669169.1_supercont1.6:1109058..1109126	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-34	UGGCAGUGUGGUUAGCUGGUUGU	23	KB669169.1_supercont1.6:1091079..1091145	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-375	UUUGUUCGUUUGGCUCGAGUUA	22	KB669281.1_supercont1.70:314839..314901	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-7	UGGAAGACUAGUGAUUUUGUUGUU	24	KB668798.1_supercont1.265:312209..312271	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-79	AUAAAGCUAGAUUACCAAAGCAU	23	KB668930.1_supercont1.384:9585..9649	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-8	UAAUACUGUCAGGUAAAGAUGUC	23	KB668666.1_supercont1.146:27512..27573	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-87	CCAGCCUGAAAUUUGCUAAACCU	23	KB669058.1_supercont1.5:469321..469384	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-927-5p	UUUAGAAUUCCUACGCUUUACC	22	KB668660.1_supercont1.140:64296..64359	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-929	AAAUUGACUCUAGUAGGGAGU	21	KB668836.1_supercont1.3:1318319..1318378	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-92a	UAUUGCACUUGUCCCGGCCUAU	22	KB668836.1_supercont1.3:1691007..1691065	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-92b	AAUUGCACUUGUCCCGGCCUGC	22	KB668836.1_supercont1.3:1704691..1704754	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-957	UGAAACCGUCCAAAACUGAGGC	22	KB669514.1_supercont1.91:212823..212889	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-970	UCAUAAGACACACGCGGCUAU	21	KB668797.1_supercont1.264:230410..230478	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-981	UUCGUUGUCGACGAAACCUGCA	22	KB668389.1_supercont1.115:58097..58172	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\surd\) \(\times\)
afu-miR-988	CCCCUUGUUGCAAACCUCACGC	22	KB669391.1_supercont1.8:63805..63867	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\times\) \(\times\) \(\times\)	\(\times\) \(\times\) \(\times\)
afu-miR-993	GAAGCUCGUUUCUAUAGAGGUAUC	24	KB668870.1_supercont1.33:220731..220822	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-996	UGACUAGAUUACAUGCUCGUCU	22	KB668289.1_supercont1.106:354280..354344	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-9a	UCUUUGGUUAUCUAGCUGUAUGA	23	KB668816.1_supercont1.281:204094..204152	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-mir-9b	ACUUUGGUGAUUUAGCUGUAUGU	23	KB668930.1_supercont1.384:9150..9215	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)	\(\surd\) \(\times\) \(\surd\)
afu-miR-9c	UCUUUGGUAUUCUAGCUGUAGA	22	KB668930.1_supercont1.384:12370..12438	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)	\(\surd\) \(\surd\) \(\surd\)
afu-miR-iab-4	ACGUAUACUGAAUGUAUCCUGA	22	KB668694.1_supercont1.171:357100..357157	\(\times\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\times\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-12	UGAGUAUUACAUCAGGUACUGGU	23	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-306	UCAGGUACUGGAUGACUCUCAG	22	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-mir-965	UAAGCGUAUAGCUUUUCCCAUU	22	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-989	UGUGAUGUGACGUAGUGGUAC	21	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-1174	UCAGAUCUACUUCAUACCCAUG	22	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)
afu-miR-1889	ACACAUUACAGAUUGGGAUUA	21	Unknown	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)	\(\surd\) \(\times\) \(\times\)

The three columns for each life stage correspond to detection of the full precursor structure namely mature, loop and star sequence, respectively. \(\surd\) \(\surd\) \(\surd\) indicates that miRNA full precursor structure was detected in the library where \(\times\) \(\times\) \(\times\) indicates the the miRNA was not detected in the library

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