Table 3 Novel miRNAs identified in the four life stages libraries of An. funestus

afu-miR-2-3

UAUCACAGCCAGCUUUGAAGAGC

23

KB668788.1_supercont1.256:85435..85508

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-2a

Identified in An. anthropophagus and An. gambiae [53, 100]

afu-miR-252

CUAAGUACUAGUGCCGCAGGAG

22

KB669170.1_supercont1.60:199766..199821

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

dme-miR-252-5p

Identified in An. anthropophagus and An. gambiae [53, 100]

afu-miR-932

UCAAUUCCGUAGUGCAUUGCAGU

23

KB668896.1_supercont1.353:66093..66159

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

tca-mir-932

Identified in An. anthropophagus and An. gambiae [53, 100]

afu-miR-2796

GUAGGCCGGCGGAAACUACUUGC

23

KB669425.1_supercont1.83:106051..106111

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

bmo-miR-2796-3p

Identified in An. anthropophagus and An. gambiae [53, 100]

afu-miR-971

UUGGUGUUAUAUCUUACAGUGAG

23

KB669081.1_supercont1.52:713511..713586

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\times\)

\(\surd\) \(\surd\) \(\surd\)

ame-miR-971

Identified in An. gambiae [53]

afu-miR-998

UAGCACCAUGAGAUUCAGCUC

21

KB669169.1_supercont1.6:410177..410245

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\times\) \(\times\) \(\times\)

aae-miR-998

Identified in An. gambiae [53]

afu-mir-2942

UAUUCGAGACCUUCACGAGUUAA

23

KB668333.1_supercont1.11:1154487..1154559

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

aae-miR-2942

Identified in An. gambiae [53]

afu-miR-31

UAGCUAUUCAACUUCUUGUUUAU

23

KB668245.1_supercont1.102:383384..383442

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

cqu-miR-31-3p

Identified in An. gambiae [100]

afu-mir-33

GUGCAUUGUAGUUGCAUUGCA

21

KB668389.1_supercont1.115:38232..38297

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-33

Identified in An. gambiae [100]

afu-miR-285

UAGCACCAUUCGAAAUCAGUAC

22

KB668666.1_supercont1.146:348761..348825

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-285

Identified in An. gambiae [100]

afu-miR-980-5p

CGGUCGUUCACCAGGUCAUCUAGC

24

KB668730.1_supercont1.203:371780..371842

\(\surd\) \(\surd\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

aae-miR-980-5p

Identified in An. gambiae [100]

afu-miR-999

UGUUAACUGUAAGACUGUGUCU

22

KB668862.1_supercont1.322:158491..158552

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

aae-miR-999

Identified in An. gambiae [100]

afu-miR-2940

GUCGACAGAGAGAUAGAUCACU

22

KB668668.1_supercont1.148:483999..484065

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-2940

Identified in An. gambiae [100]

afu-miR-2944a

GAAGGAACUUCUGCUGUGAUCU

22

KB668445.1_supercont1.120:344303..344359

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-2944a-5p

Identified in An. gambiae [100]

afu-miR-2944b

GAAGGAACUCCCGGUGUGAUAUA

23

KB668456.1_supercont1.121:76523..76595

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-2944b-5p

Identified in An. gambiae [100]

afu-miR-71

UCUCACUACCUUGUCUUUCAUG

22

KB668788.1_supercont1.256:83429..83494

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

aae-miR-71-3p

Novel in Anopheles spp.

afu-miR-193

UACUGGCCUACUAAGUCCCAAC

22

KB668718.1_supercont1.193:152692..152757

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\times\) \(\times\) \(\times\)

aae-miR-193

Novel in Anopheles spp.

afu-miR-316

UGUCUUUUCCUGCUUACUGCCG

22

KB668714.1_supercont1.19:760291..760355

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

aae-miR-316

Novel in Anopheles spp.

afu-miR-927-3p

UAAAGCGUAGGAAUUCUAAAAC

22

KB668660.1_supercont1.140:64294..64357

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

dme-miR-927-5p

Novel in Anopheles spp.

afu-miR-980-3p

UAGCUGCCUAGUGAAGGGC

19

KB668730.1_supercont1.203:371784..371839

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

aae-miR-980-3p

Novel in Anopheles spp.

afu-miR-2765

UGGUAACUCCACCACCGUUGGC

22

KB668981.1_supercont1.43:346454..346513

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

aae-miR-2765

Novel in Anopheles spp.

afu-miR-2941

UAGUACGGAUAAGUAACACACU

22

KB668992.1_supercont1.44:163365..163440

\(\surd\) \(\surd\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

aae-miR-2941

Novel in Anopheles spp.

afu-miR-2943

UUAAGUAGGCACUUGCAGGCAA

22

KB668790.1_supercont1.258:358408..358469

\(\surd\) \(\surd\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

aae-miR-2943

Novel in Anopheles spp.

afu-miR-a

UGAGGUAAACCGCGCUUAAAGA

22

KB668367.1_supercont1.113:186839..186900

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-b

AACGGACGGGUACCUUCGCACC

22

KB668478.1_supercont1.123:544003..544072

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-c

UCAACAUACAUUCUCGUUCUGU

22

KB668522.1_supercont1.127:174494..174560

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-d

UAAUACAUUUGCUUACGGCAGAGU

24

KB668522.1_supercont1.127:49592..49655

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-e

UCUGCCGUAGGAAUGUAUUUACC

23

KB668522.1_supercont1.127:51191..51253

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-f

UGCUGUAGGAAGUGAUUUACCU

22

KB668522.1_supercont1.127:51545..51607

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-g

AGUCAUCGUCGUCACUCGAUCGA

23

KB668728.1_supercont1.201:241585..241642

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-h

CCAUCUGACGGACACCACCGGA

22

KB668739.1_supercont1.211:143895..143961

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-i

AUGGCAGUCAACGUAUACCCAUU

23

KB668781.1_supercont1.25:515317..515377

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-j

UUCCGAGUGGACAAGUGGAACCU

23

KB668806.1_supercont1.272:68360..68416

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-k

UACCUGAGUUCGUUUAAACUGA

22

KB668821.1_supercont1.286:244740..244793

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

-

Novel

afu-miR-l

UCUAUCAUUUGAGUACCAUGA

21

KB668837.1_supercont1.30:192923..192989

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-m

CUAACCUGUAAGGAGCUUUGGCGGC

25

KB668906.1_supercont1.362:184842..184933

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

-

Novel

afu-miR-n

AUGGCACAAGCAACAUCAGCUG

22

KB668909.1_supercont1.365:72126..72172

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

-

Novel

afu-miR-o

UCUUGAGCGUAUUUGGCACUGCU

23

KB668992.1_supercont1.44:163580..163641

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\times\) \(\times\) \(\times\)

\(\surd\) \(\times\) \(\times\)

-

Novel

afu-miR-p

UAGCUCAACAAACAUCUGGAGGA

23

KB669059.1_supercont1.50:521609..521669

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel

afu-miR-q

UACAACCGGACGGUACACUGCAUAG

25

KB669214.1_supercont1.64:671164..671237

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-r

AACGAAGUUGAUCCGCUGAAGCU

23

KB669480.1_supercont1.88:541508..541571

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-s

ACUUGAAACGUAGUCCGGGAACCC

24

KB669502.1_supercont1.9:563831..563898

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

\(\times\) \(\times\) \(\times\)

-

Novel

afu-miR-t

AUUAGAAUGUGGAAUCUGUUUUU

23

KB668820.1_supercont1.285:93655..93723

\(\surd\) \(\surd\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

\(\surd\) \(\times\) \(\surd\)

-

Novel