Fig. 2

Normalized dose–response curve for the competitive binding assay. Binding of (a) 17A and (b) 21A to the ATP binding domain of PfHsp90 was assessed using the bis-ANS competitive binding assay. Reduction in the fluorescence of bis-ANS was measured using a spectrophotometer. The average IC₅₀ values were calculated from three experiments using Graphpad Prism 6. The mean fluorescence and standard error of the mean (SEM) of duplicate wells is shown. Each graph is a representative of three independent experiments