Fig. 4
a Conformation of the cloned pET28b vector containing the Plasmodium vivax parasite glutamate dehydrogenase (pGDH) gene (pVKtype3). M, molecular size marker; lane 1 undigested plasmid; lane 2 NdeI-digested plasmid; lane 3 NdeI- and XhoI-digested plasmid. b Expression of the pGDH gene in Escherichia coli BL21(DE3)pLysS. Lane M molecular weight protein marker; lane 1, Non-induction clone, lanes 2–4 induction clones of recombinant pGDH protein. c Purification of the pGDH protein from Escherichia coli BL21(DE3)pLysS. Lane M molecular weight protein marker; lane 1 purified recombinant pGDH protein