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Table 1 Sequences of primers and probe used for the quantitative PCR detection of P. berghei in mosquitoes

From: MALDI-TOF MS as an innovative tool for detection of Plasmodium parasites in Anopheles mosquitoes

Target

Primer name

Sequence

18S gene

All Plasmodium species

18S_F

5′-AGGCAACAACAGGTCTGTGA-3′

18S_R

5′-GCAATAATCTATCCCCATCACG-3′

18S_P

5′-6FAM- GAACTAGGCTGCACGCGTGCTACA-3′

  1. Primers and probe targeting a 189 bp sequence of all Plasmodium species 18S gene used for the detection of Plasmodium berghei ANKA in mosquitoes