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Table 4 Comparison of molecular biology and mass spectrometry performances for the detection of Plasmodium berghei in An. stephensi mosquitoes

From: MALDI-TOF MS as an innovative tool for detection of Plasmodium parasites in Anopheles mosquitoes

  Controls PCR+ PCR- TOTAL
Total number of spectra 42 20 38 100
Number of spectra introduced in DB 12 6 2 20
Number of spectra tested blinded against DB 30 14 36 80
Percentage of MALDI-TOF consistency with PCR 30/30 (100%) 13/14 (92.8%) 36/36 (100%) 79/80 (98.75%)
  1. Results of MALDI-TOF blinded tests were based on the highest log score values and obtained with the MALDI BioTyper software. Controls: Anopheles fed on non-infected mice, PCR + : mosquitoes fed on infected mice and for which P. berghei-PCR was positive, PCR − : mosquitoes fed on infected mice and for which P. berghei-PCR was negative. DB database