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Fig. 3 | Malaria Journal

Fig. 3

From: Profile of molecular mutations in pfdhfr, pfdhps, pfmdr1, and pfcrt genes of Plasmodium falciparum related to resistance to different anti-malarial drugs in the Bata District (Equatorial Guinea)

Fig. 3

Electrophoresis in agarose gel of the RFLPs for position 164 in pfdhfr. Electrophoresis of the result of the digestion with the PsiI enzyme. It can be seen that in two samples (12N and 119_02_03) there is no digestion, indicating that the position 164 is mutated (164L) and the enzyme cannot recognize its target. When is digested the fragment of 254 bp, appear two fragments 214 and 42 bp. C− Control of “non-digested”, fragment of PCR (size 254 bp) that was not subjected to digestion with PsiI enzyme. C+ Control of digestion, fragment of the PCR that always is digested with the PsiI enzyme

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