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Table 1 Primers used for the amplification of PvMCA1-cd, pvmdr1, and Pvcsp of P. vivax isolates

From: Genetic diversity of Plasmodium vivax metacaspase 1 and Plasmodium vivax multi-drug resistance 1 genes of field isolates from Mauritania, Sudan and Oman

Genes Primers Sequences PCR program
PvMCA1-cd MCA1-F1 5′-CATGGAAACAAAAAAAAGG-3′ 95 °C 10′, 35 cycles 94 °C 30″ 56 °C 30″ 72 °C 2′
MCA1-R1 5′-CGAAAACTCCATATCTTTGC-3′
pvmdr1 mdr1-F1 5′-ATAGTCATGCCCCAGGATTG-3′ 95 °C 10′, 45 cycles 95 °C 10″, 60 °C 10″ and 72 °C 30″
mdr1-R1 5′-ACGTTTGGTCTGGACAAGTAT-3′
Pvcsp VCSP-OF 5′-ATGTAGATCTGTCCAAGGCCATAAA-3′ Primary reaction
VCSP-OR 5′-TAATTGAATAATGCTAGGACTAACAATATG-3′ 95 °C 10′, 40 cycles 95 °C 1′, 58 °C 2′ and 72 °C 2′
VCSP-NF 5′-GCAGAACCAAAAAATCCACGTGAAAATAAG-3′ Nested reaction
VCSP-NR 5′-CCAACGGTAGCTCTAACTTTATCTAGGTAT-3′ 30 cycles 95 °C 1′, 62 °C 2′ and 72 °C 2′
  1. PvMCA1-cd: catalytic domain of Metacaspase1’s P. vivax
  2. Pvmdr1: multidrug-resistant of P. vivax
  3. Pvcsp: circumsporozoite surface protein gene of P. vivax
  4. ’: minute
  5. ”: second
  6. °C: degree Celsius