Expression of 6-Cys domain proteins: Pfs41, Pfs38, Pfs12 and PfMSP-165. a Schematic showing the organization of the above-mentioned Plasmodium proteins. Arrows mark the regions that were expressed in Escherichia coli.
b Coomassie stained SDS-PAGE and immunoblots showing the purified Pfs41, Pfs38, Pfs12 and PfMSP-165. Immunoblots were performed using mouse monoclonal anti-his antibody. c Recognition of native Pfs41, Pfs38 and Pfs12 proteins in parasite lysate from asexual blood stages using their respective antibodies generated against recombinant proteins