Fig. 3

Nonsynonymous and copy number variant analyses to determine the drug target of anti-malarial drug SJ733. The relative copy number for 3000 nucleotide tiles across the entire P. falciparum genome are mapped across all chromosomes (top panel). The read support for susceptible parent (black) and resistant mutant (blue) strains show a relatively even distribution of sequencing reads. Zooming into a reanalysis of chromosome 12 with 300 nucleotide tiles (middle panel) shows spurious copy number data in areas of relatively low read coverage. Zooming further in (bottom panel) shows that the variation that encodes P412T in ATPase4 (arrow) is present in 72 of 74 reads in the resistant mutant strain, but only 3 in 684 of the susceptible parent strain. This nonsynonymous mutation determines resistance to SJ733. Each panel is to scale