Fig. 4

Nonsynonymous and copy number variant analyses to determine the drug target of apicoplast-specific inhibitor MMV-08138. Depictions match Fig. 3. These data show no significant copy number variation. The bottom panel shows that the variation that encodes L244I in IspD (arrow) is present in 358 of 362 overlapping reads from the resistant mutant strain, but absent in all 242 from the susceptible parent strain. This nonsynonymous mutation determines resistance to MMV-08138