Selections, frameshift mutations, and copy number variation detected on the surf 4.1 gene in the western Kenyan Plasmodium falciparum population

Table 2 Test of neutrality for Plasmodium falciparum surf _4.1 for the western Kenyan isolates (n = 24)

Region	Nucleotide position	Number of sites (base)	η	S	Two variants		More than two variants	π	θ	Tajima’s D	Fu and Li’s
Region	Nucleotide position	Number of sites (base)	η	S	Singleton	Not singleton	More than two variants	π	θ	Tajima’s D	D*	F*
Extracellular	4–2289	2286	439	410	115	266	29	0.046	0.048	−0.19	−0.29	−0.30
Nter	4–150	147	2	2	1	1	0	0.002	0.004	−1.20	−0.66	−0.93
CRD	151–585	435	11	10	3	6	1	0.007	0.006	0.39	−0.61	−0.36
Var1	586–1506	918	109	107	44	61	2	0.022	0.031	−1.17	−0.93	−1.18
Var2	1507–2289	783	317	291	67	198	26	0.104	0.100	0.17	−0.02	0.05

Sequence number is after 3D7 line sequence
Extracellular, extracellular region; Nter, N-terminal segment; CRD, cysteine-rich domain; Var1, variable region 1; Var2, variable region 2; sites, nucleotide sites analyzed; η, the total number of mutations; S, number of segregating sites; π, observed nucleotide diversity; θ, the expected nucleotide diversity under neutrality derived from S

ISSN: 1475-2875