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Fig. 1 | Malaria Journal

Fig. 1

From: Tryptophan-rich domains of Plasmodium falciparum SURFIN4.2 and Plasmodium vivax PvSTP2 interact with membrane skeleton of red blood cell

Fig. 1

Localization and signal intensity comparison of mini-SURFIN4.1, NTC-GFP and NTC-4.2WRD2-GFP, in P. falciparum-iRBC. a Co-localization of NTC-GFP with the PVM marker EXP2 and Maurer’s cleft marker SBP1. Upper panel Schematic drawing of NTC-GFP. Lower panels Representative fluorescence images showing the co-localization of NTC-GFP with EXP2 and SBP1. b Co-localization of NTC-4.2WRD2-GFP with EXP2, SBP1 and PfEMP1. Upper panel Schematic drawing of NTC-4.2WRD2-GFP. Lower panels Representative fluorescence images showing the co-localization of NTC-4.2WRD2-GFP with EXP2, SBP1 and PfEMP1. The differential interference contrast images merged with nucleus stained with DAPI (DIC + Nuc), fluorescence images with mouse anti-GFP antibody (anti-GFP), PVM location with rabbit anti-EXP2 antibody (anti-EXP2) or Maurer’s cleft location with rabbit anti-SBP1 antibody (anti-SBP1), or rat anti-PfEMP1 antibody (anti-PfEMP1), and merged image are shown. Bar 5 μm. Scale refers to the residue number of recombinant SURFIN4.1 protein. Plot profiles of signal intensities evaluated by ImageJ software are shown by a grey scale on the right side of each immunofluorescence panel, along with the Western blot data for recombinant SURFINs. The bands at the predicted size for the recombinant NTC-GFP and NTC-4.2WRD2-GFP are marked with arrowheads. Parasite/PV indicates parasite cytosol or parasitophorous vacuole

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