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Fig. 3 | Malaria Journal

Fig. 3

From: Tryptophan-rich domains of Plasmodium falciparum SURFIN4.2 and Plasmodium vivax PvSTP2 interact with membrane skeleton of red blood cell

Fig. 3

Interactions of recombinant WRDs of SURFIN4.2 and PvSTP2 with IOVs. a His-tagged recombinant WRD proteins were purified using the Ni-IDA-Sefinose TM Resin and analysed by Western blot. S Soluble fraction, P pellet fraction. Arrowheads indicate bands of His-tagged recombinant PvSTP2WRD (calculated MW, 46.5 kDa), SURFIN WRD14.2 (48.9 kDa), SURFIN WRD34.2 (51.0 kDa), and Pf332WRD (46.5 kDa). Soluble fractions of each recombinant protein were used for the in vitro interaction assays with membrane skeleton of RBC. b Saturation curves of the binding of recombinant WRD proteins to IOVs were measured by ELISA-based protein interaction analysis

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Malaria Journal

ISSN: 1475-2875

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