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Fig. 4 | Malaria Journal

Fig. 4

From: Tryptophan-rich domains of Plasmodium falciparum SURFIN4.2 and Plasmodium vivax PvSTP2 interact with membrane skeleton of red blood cell

Fig. 4

Binding of the His-tagged recombinant SURFIN WRD24.2 to F-actin. a The supernatant and pellet fractions of SURFIN WRD24.2 -His (SURFIN WRD24.2 ), Pf332WRD-His (Pf332WRD), and His-tag control protein after F-actin co-sedimentation assay were analysed by Western blot with anti-His tag or anti-actin antibodies. Arrows indicate recombinant protein bands with the expected size. S Supernatant fraction, P pellet fraction. b Western blot of the supernatant (left) and pellet (right) fractions after F-actin co-sedimentation with serially diluted SURFIN WRD24.2 -His, control His-tagged control protein, and Pf332WRD-His are shown. Because the signal of the Pf332WRD detected in the pellet fraction was saturated, the intensity of this lane was reduced. c Saturation curves of the binding of SURFIN WRD24.2 -His to F-actin based on the F-actin co-sedimentation assays

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