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Table 4 Gametocyte detection by Pvs25 mRNA based detection (RT-PCR and qRT-PCR) in 210 P. vivax-positive samples and their overlaps with parasite detection by other methods

From: Comparison of methods for detecting asymptomatic malaria infections in the China–Myanmar border area

qRT-PCR (Pvs25)

LM

CLIP-PCR

nD-PCR

nRT-PCR

RT-PCR (Pvs25)

Total

Positive

Negative

Positive

Negative

Positive

Negative

Positive

Negative

Positive

Negative

Positive

7

108

11

104

11

104

87

28

61

54

115

Negative

5

90

8

87

13

82

95

0

0

95

95

Total

12

198

19

191

24

186

182

28

61

149

210

Gametocyte carriers (%)

7/12 (58.33%)

11/19 (57.89%)

11/24 (45.83%)

87/182 (47.80%)

61/61 (100%)

115/210 (54.76%)

RT-PCR (Pvs25)

LM

CLIP-PCR

nD-PCR

nRT-PCR

qRT-PCR (Pvs25)

 

Positive

7

54

7

54

9

52

33

28

61

0

61

Negative

5

144

12

137

15

134

149

0

54

95

149

Total

12

198

19

191

24

186

182

28

115

95

210

Gametocyte carriers (%)

7/12 (58.33%)

7/19 (36.84%)

9//24 (37.50%)

33/182 (18.13%)

61/115 (53.04%)

61/210 (29.05%)