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Table 4 Gametocyte detection by Pvs25 mRNA based detection (RT-PCR and qRT-PCR) in 210 P. vivax-positive samples and their overlaps with parasite detection by other methods

From: Comparison of methods for detecting asymptomatic malaria infections in the China–Myanmar border area

qRT-PCR (Pvs25) LM CLIP-PCR nD-PCR nRT-PCR RT-PCR (Pvs25) Total
Positive Negative Positive Negative Positive Negative Positive Negative Positive Negative
Positive 7 108 11 104 11 104 87 28 61 54 115
Negative 5 90 8 87 13 82 95 0 0 95 95
Total 12 198 19 191 24 186 182 28 61 149 210
Gametocyte carriers (%) 7/12 (58.33%) 11/19 (57.89%) 11/24 (45.83%) 87/182 (47.80%) 61/61 (100%) 115/210 (54.76%)
RT-PCR (Pvs25) LM CLIP-PCR nD-PCR nRT-PCR qRT-PCR (Pvs25)  
Positive 7 54 7 54 9 52 33 28 61 0 61
Negative 5 144 12 137 15 134 149 0 54 95 149
Total 12 198 19 191 24 186 182 28 115 95 210
Gametocyte carriers (%) 7/12 (58.33%) 7/19 (36.84%) 9//24 (37.50%) 33/182 (18.13%) 61/115 (53.04%) 61/210 (29.05%)