Comparison of methods for detecting asymptomatic malaria infections in the China–Myanmar border area

Malaria Journal

Table 4 Gametocyte detection by Pvs25 mRNA based detection (RT-PCR and qRT-PCR) in 210 P. vivax-positive samples and their overlaps with parasite detection by other methods

qRT-PCR (Pvs25)	LM		CLIP-PCR		nD-PCR		nRT-PCR		RT-PCR (Pvs25)		Total
qRT-PCR (Pvs25)	Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative	Positive	Negative	Total
Positive	7	108	11	104	11	104	87	28	61	54	115
Negative	5	90	8	87	13	82	95	0	0	95	95
Total	12	198	19	191	24	186	182	28	61	149	210
Gametocyte carriers (%)	7/12 (58.33%)		11/19 (57.89%)		11/24 (45.83%)		87/182 (47.80%)		61/61 (100%)		115/210 (54.76%)

RT-PCR (Pvs25)	LM		CLIP-PCR		nD-PCR		nRT-PCR		qRT-PCR (Pvs25)
Positive	7	54	7	54	9	52	33	28	61	0	61
Negative	5	144	12	137	15	134	149	0	54	95	149
Total	12	198	19	191	24	186	182	28	115	95	210
Gametocyte carriers (%)	7/12 (58.33%)		7/19 (36.84%)		9//24 (37.50%)		33/182 (18.13%)		61/115 (53.04%)		61/210 (29.05%)

ISSN: 1475-2875