Table 2 Primers and PCR conditions for the amplification of different drug resistance loci

Pfcrt SNPs at codons 72 and 76

OF P1

OR P2

5′-CCGTTAATAATAAATACACGCG-3′

5′-CGGATGTTACAAAACTATAGTCC-3′

35 cycles of 94 °C for 30 s; 56 °C for 30 s; and 62 °C for 1 min

Mekonnen et al. [11]

NF P3

NR P4

5′-AGGTTCTTGTCTTGGTAAATTTGC-3′

5′-CAAAACTATAGTTACCAATTTTG-3′

30 cycles of 94 °C for 30 s; 56 °C for 30 s; and 65 °C for 1 min

Pfmdr1 SNPs at codon 86 and 184

OF P5

OR P6

5′-AGGTTGAAAAAGAGTTGAAC-3′

5′-ATGACACCACAAACATAAAT-3′

30 cycles of 94 °C for 30 s; 55 °C for 30 s; and 65 °C for 1 min

NF P7

NR P8

5′-ACAAAAAGAGTACCGCTGAAT-3′

5′-AAACGCAAGTAATACATAAAGTC-3′

30 cycles of 94 °C 30 s; 60 °C for 30 s; and 65 °C for 1 min

Pfmdr1 SNPs at codon 1034,1024, and 1246

OF P9

OR P10

5′-GTGTATTTGCTGTAAGAGCT-3′

5′-GACATATTAAATAACATGGGTTC-3′

34 cycles of 94 °C for 30 s; 55 °C for 1 min and 72 °C for 2 min

NF P11

NR 12

5′-CAGATGATGAAATGTTTAAAGATC-3′

5′-TAAATAACATGGGTTCTTGACT-3′

29 cycles of 94 °C for 30 s; 60 °C for 30 s; and 65 °C for 1 min

Pfatp6 at codon 243, 263 and 431

NF P13

NR P14

5′-TCATCTACCGCTATTGTATG-3′

5′-TCCTCTTAGCACCACTCC-3′

35 cycles of 94 °C for 45 s; 55 °C for 1 min; 72 °C for 1 min

Zakeri et al. [24]

Pfatp6 SNPs at codon 623 and 769

NF P15

NR P16

5′-TGGAGACAGTACCGAATTAGC-3′

5′-TCTTCCTACATATTTACGTGGTG-3′

Pfk13

OF P17

OR P18

5′-CGGAGTGACCAAATCTGGGA-3′

5′-GGGAATCTGGTGGTAACAGC-3′

30 cycles of 95 °C for 30 s; 58 °C for 2 min; 72 °C for 2 min

Ariey et al. [16]

NF P19

NR P20

5′-GCCAAGCTGCCATTCATTTG-3′

5′-GCCTTGTTGAAAGAAGCAGA-3′

40 cycles of 95 °C for 30 s; 60 °C for 1 min; 72 °C for 1 min