Fig. 1

Schematic diagram, recombinant protein expression, purification, and western blot analysis of rPvMSP8. a Schematic diagram of PvMSP8. The PvMSP8 protein comprises 487 amino acids, with a calculated molecular mass of 54.7 kDa. The GPI anchor (amino acid [aa] position 464–486), the EGF domain (aa 383–463), the asparagine-rich region (ASN, aa 25–123), and the signal peptide (aa 1–23) are indicated. A truncated PvMSP8 (aa 24–463) was constructed for expression. T, total; S, soluble; P, pellet; Ft, flow-through; E, elution. b The purification progress of rPvMSP8 (55 kDa) was resolved by 12.5% SDS-PAGE. c Western blot analysis of rPvMSP8 using an anti-Penta-His antibody (H), healthy individuals (HI), mixed vivax patient sera (Pt), pre-immune sera (PI), and mouse immune sera (M), respectively. Arrowheads indicate specific bands for each recombinant protein. M, molecular size marker