Fig. 3

Total IgG and IgG subclass responses to rPvMSP8. a rPvMSP8 was probed with the sera of 112 malaria patients and 80 healthy individuals from the ROK. Significant differences were observed between vivax patients and healthy individuals in the total prevalence of anti-rPvMSP8 IgG (P < 0.0001). b Comparison of the IgG responses to PvMSP8 among the Korea, Myanmar and Thailand. Significant differences were observed between vivax patients from Korea, Myanmar and Thailand and healthy individuals in the IgG prevalence of anti-rPvMSP8 IgG (P < 0.0001). c The immunoreactivity of IgG subclass antibodies against rPvMSP8 using the sera of 50 patients randomly selected from those described above was also determined. The cutoff value was defined as two standard deviations (SDs) above the mean fluorescence intensity of eight negative control samples. The error bars indicate the mean ± SD. d IgG subclass levels in immune mice. The results are expressed as mean concentrations ± SDs