Fig. 1From: Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAsSpecific amplicon produced by RT-PCR targeting gametocyte-specific spliced PF3D7_0630000 mRNA. a RT-PCR was performed with (+RT) and without (−RT) reverse transcriptase on nucleic acids extracted from mature gametocytes (GAM) or from asexual-stage infected red blood cells (ASEX) and products were separated by 1% agarose gel electrophoresis. a 100 bp ladder is shown at left. b PF3D7_0630000 mRNABack to article page