Fig. 2
Specific detection of spliced gametocyte-specific mRNAs by hydrolysis probe RT-PCR. RT-PCR was performed using hydrolysis probes for the four spliced gametocyte-specific targets listed on nucleic acids extracted from asexual-stage infected red blood cells (left panels) or from mature gametocytes (right panels). Reactions were multiplexed with a primer/probe set for the Plasmodium 18S rRNA. Horizontal lines denote the RT-PCR thresholds used to calculate CT values (lower green line 18S rRNA; upper blue line candidate mRNA). Y-axis, RFU (raw fluorescence units) from Biorad C1000/CFX instrument. ND not detected