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Table 2 Cycle thresholds for Plasmodium 18S rRNA, PF3D7_0630000 and pfs25 in gametocyte dilution series

From: Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Gametocytes/mL

Plasmodium 18S rRNA

PF3D7_0630000

pfs25

3 × 107

5.40

15.70

10.53

3 × 106

9.36

19.70

14.48

8.85

19.38

13.94

3 × 105

12.16

23.13

17.91

12.34

22.98

17.42

3 × 104

15.12

26.47

20.70

15.11

26.50

20.65

3 × 103

18.14

29.43

23.60

18.63

29.60

23.99

3 × 102

21.85

33.86

27.74

21.82

33.65

27.92

3 × 101

24.06

37.89

30.35

ND

ND

ND

3 × 100

ND

ND

ND

ND

ND

ND

  1. Plasmodium 18S rRNA RT-PCR LOD = 20 parasites/mL
  2. ND, not detected; All in duplicate except 3 × 107 gametocyte/mL sample
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Malaria Journal

ISSN: 1475-2875

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