Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs

Table 3 Example cycle thresholds for PF3D7_0630000 and published gametocyte-specific RT-PCRs

RT-PCR target (annealing temp.)	Asexual		Gametocyte 3 × 10⁶ g/mL	Negative control
RT-PCR target (annealing temp.)	4 × 10⁵ p/mL	8 × 10² p/mL	Gametocyte 3 × 10⁶ g/mL	Negative control
PF3D7_0630000 (50 °C)	ND	ND	23.02	ND
PF3D7_0816800 (50 °C)	ND	ND	24.98	ND
PF3D7_1477700 (50 °C)	31.96	ND	25.99	ND
PF3D7_1477700 (60 °C)	31.67	38.74	26.50	ND
PF3D7_1438800 (50 °C)	35.42	ND	24.94	ND
PF3D7_1438800 (60 °C)	33.48	ND	25.51	ND
pfs25 (50 °C, DNased template)	ND	ND	17.67	ND
Plasmodium 18S rRNA	14.91	23.82	10.20	ND

ISSN: 1475-2875