Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

Table 2 Summary of sequenced DNA samples (positive controls, Kenya and Solomon Islands) based on Plasmodium COX-I gene

Sample	Species ID^a	E value^b	Coverage^c (%)	Identity^d	[GenBank identifier]^e
PCR product from dilution 6 (0.043 pg of DNA)
P. falciparum	P. falciparum	0	100	99.7%	[KM065500.1]
P. vivax	P. vivax	1.3e–84	100	98.4%	[KF668441.1]
P. knowlesi	P. knowlesi	8e–180	100	100%	[AB444108.1]
Known infective mosquitoes from the field (Kenya Highlands)
Mosquitoes (n = 21)	P. falciparum	0	100	99.7%	[KM065500.1]
Mosquitoes (n = 3)	P. ovale s.l.	1.21e–151	100	97.5%	[KF018660.1]
Positive mosquitoes from Western Province, Solomon Islands
Mosquitoes (n = 17)	P. falciparum	0	100	99.7%	[KM065500.1]
Mosquitoes (n = 5)	P. vivax	0	99	99.6	[KF668441.1]
Mosquito (n = 1)	P. ovale wallikeri	7.1e–110	100	92.2%	[HQ712053.1]

ISSN: 1475-2875