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Table 4 Summary of cost analysis for the 18s-rRNA nested and COX-I PCRs for Plasmodium spp. detection

From: Fast and robust single PCR for Plasmodium sporozoite detection in mosquitoes using the cytochrome oxidase I gene

PCR technique Required PCR reagents Estimated cost of the PCR kit µL of polymerase used per reaction Cost of PCR diagnosis per sample Cost of 2122 reactions for Plasmodium detection
18s-rRNA nested PCR Taq polymerase kit (Invitrogen) ~210 USD 0.1 µL for nest-1 and 0.1 µL nest-2 ~0.42 USD ~892 USD
Conventional COX-I single PCR Taq polymerase kit (Invitrogen) ~210 USD 0.2 µL in a single reaction ~0.42 USD ~892 USD
Fast COX-I single PCR Blood Phusion polymerase kit (Thermo) ~418 USD 0.125 µL in a single reaction ~0.26 USD ~552 USD