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Table 3 Distribution of mutants using pools from individually extracted (1×), 3-punch extracted (3×) and 10-punch extracted (10×)

From: Surveillance for sulfadoxine-pyrimethamine resistant malaria parasites in the Lake and Southern Zones, Tanzania, using pooling and next-generation sequencing

DNA pool Proportion of mutant alleles
Dhps540 Dhps581 Dhfr164
88.5% (339/383) 2.8% (13/464) 0% (0/319)
88.6% (1014/1145) 3.8% (52/1380) 0.3% (2/586)
10× 89.9% (563/626) 3.3% (25/765) 0% (0/401)