Surveillance for sulfadoxine-pyrimethamine resistant malaria parasites in the Lake and Southern Zones, Tanzania, using pooling and next-generation sequencing

Table 3 Distribution of mutants using pools from individually extracted (1×), 3-punch extracted (3×) and 10-punch extracted (10×)

DNA pool	Proportion of mutant alleles
DNA pool	Dhps540	Dhps581	Dhfr164
1×	88.5% (339/383)	2.8% (13/464)	0% (0/319)
3×	88.6% (1014/1145)	3.8% (52/1380)	0.3% (2/586)
10×	89.9% (563/626)	3.3% (25/765)	0% (0/401)

ISSN: 1475-2875