Fig. 1

Pfs25 expression on Hela cells. Hela cells were transfected with an Ad5 shuttle vector with (a) or without (b) the codon-optimized Pfs25 gene under the control of the CMV immediate early promotor. Twenty-four hours post transfection, cells were fixed, permeabilized, and probed with ID2, a conformation-dependent Pfs25-specific monoclonal antibody, then probed with an Alexa Fluor488-conjugated anti-mouse secondary antibody and visualized by IFA