Fig. 6

Vaccination induced transmission-blocking activity. Two independent sets of mice were immunized with the test groups, yPfs25 homologous prime boost (yPfs25-alum/yPfs25-alum) or the 3 heterologous prime boost strategies (Ad5-pfs25/yPfs25-alum, Ad5-pfs25/Ad5-HVR5D2, and Ad5-pfs25/Ad5-HVR5D3) as described in "Methods". Serum was pooled from the test groups from each immunization (n = 3 mice for all the immunization groups except the 2nd yPfs25 homologous prime boost immunization, which included 6 mice) and tested in SMFAs at a 1:10 dilution. Pooled serum from mice immunized with an Ad5-luciferase vector was the negative control in each assay. The first SMFA tested pooled serum from the first immunization, while the second and third SMFAs tested pooled serum from the second immunization. All three SMFAs were included in the statistical analysis. The best estimate of the percent inhibition from 3 independent SMFAs is plotted with the 95% confidence interval for each sample calculated using a zero-inflated negative binomial model [30] (***p < 0.001, *p < 0.05 as compared to baseline)