Table 1 Primer sequences for recombineering
Primer name | Sequence |
|---|---|
Primers for galk insertion | |
 HVR1 galk forward | gtgccccaaatccttgcgaatgggatgaagctgctactgctcttgaaatacctgttgacaattaatcatcggca |
 HVR1 galk reverse | agaataaggcgcctgcccaaatacgtgagttttttgctgctcagcttgcttcagcactgtcctgctcctt |
 HVR5 galk forward | agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcacctgttgacaattaatcatcggca |
 HVR5 galk reverse | gtgtctggggtttctatatctacatcttcactgtacaataccactttaggtcagcactgtcctgctcctt |
Primers for pfs25 epitope insertion | |
 HVR1 DIII forward | gtgccccaaatccttgcgaatgggatgaagctgctactgctcttgaaataatctggatacatctaatcccgtgaagactggagtctgcagt |
 HVR1 DIII reverse | agaataaggcgcctgcccaaatacgtgagttttttgctgctcagcttgctcacaactgcagactccagtcttcacgggattagatgtatc |
 HVR5 pfsDII forward | agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcaattgatgggaacccagtgtcctacgcctgcaagtgtaat |
 HVR5 pfsDII reverse | gtgtctggggtttctatatctacatcttcactgtacaataccactttaggattacacttgcaggcgtaggacactgggttcccatcaat |
 HVR5 pfsDIII forward | agcaacaaaatggaaagctagaaagtcaagtggaaatgcaatttttctcactggatacatctaatcccgtgaagactggagtctgcagt |
 HVR5 pfsDIII reverse | Gtgtctggggtttctatatctacatcttcactgtacaataccactttaggacaactgcagactccagtcttcacgggattagatgtatc |