Fig. 1

Establishment of fumarate hydratase (FH)- and malate:quinone oxidoreductase (MQO)-deficient P. berghei. a Expression of luciferase (Luc2) and fh in control and Δfh parasites. b Expression of Luc2 and mqo in control and Δmqo parasites. The genes were subjected to semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) using specific primers (see Additional files 2, 3). hsp70 was used as a positive control. Samples treated with DNase-treated RNA template [hsp70 (−)] were used as a negative control that is the control of eventual DNA contamination of the RNA preparations. c FH activity in infected (left) and uninfected (right) erythrocytes. d MQO activity in infected erythrocytes. Results are expressed as mean ± SD of triplicate determinations