Fig. 2

Parasite growth is delayed by deficiency of FH and MQO. a C57BL/6 mice were infected with 5 × 10⁶ to 5 × 10⁷ schizonts of control, Δfh and Δmqo parasites. At 6, 12, 18 and 24 h after inoculation, the proportion of asexual-blood-stage forms in 100 infected erythrocytes was determined. Results are expressed as mean ± SD of three mice. Asterisks indicate statistically significant differences (*, vs. control parasites). b Absolute luciferase activity values in trophozoites of wild-type (WT), control, Δfh and Δmqo parasites. Trophozoites (5 × 10⁶) were obtained from C57BL/6 mice at 8 and 16 h post-inoculation with purified schizonts. Results are expressed as mean ± SD absolute luciferase activity values of three wells at 20 min after addition of d-luciferin. Experiments were performed in duplicate, and representative data are shown. Asterisks indicate statistically significant differences (**, vs. control and Δfh parasites)