Fig. 2

T cell and antibody responses induced by a single immunization of HuAd5-PyCSP, GC44-PyCSP, GC45-PyCSP or GC46-PyCSP. BALB/c mice (6 mice/group) were immunized with three different doses (1 × 10⁷, 1 × 10⁸ or 1 × 10⁹ pu) of HuAd5 Null, HuAd5-PyCSP, GC44-PyCSP, GC45-PyCSP or GC46-PyCSP. a CD8⁺ T cell IFN-γ responses were assessed 21 days after immunization by ICS. Splenocytes were stimulated with A20.2J cells loaded with peptides encoding the PyCSP immunodominant CD8⁺ T cell epitope (amino acids 280–288), the PyCSP immunodominant CD4⁺/nested CD8⁺ subdominant T cell epitopes (amino acids 57–70) or the influenza hemagglutinin CD8⁺ T cell epitope (amino acids 332–240). Error bars indicate standard errors of the means (n = 6). *p <0.05 compared with HuAd5 at the same dose. b PyCSP-specific antibody responses were assessed 21 days after immunization by ELISA. The capture antigen was purified PyCSP protein. The values for individual mice are shown (n = 6). The mean is indicated by the black line. *p <0.05 compared with HuAd5 at the same dose