Fig. 5

T cell responses induced by the prime-boost vaccines in protection study 1. IFN-γ ELISpot and ICS responses were assessed 12 days after the adenovirus boost. a IFN-γ ELISpot. Splenocytes from group pools (200,000 cells/well) were stimulated with A20.2J cells loaded with peptides encoding the PyCSP immunodominant CD8⁺ T cell epitope (amino acids 280–288) or the PyCSP CD4⁺ immunodominant/nested CD8⁺ subdominant T cell epitopes (amino acids 57–70). Spot forming cells (SFC)/1 × 10⁶ splenocytes are presented. Bars represent the mean ± standard deviation from quadruplicate wells. b ICS. Splenocytes from individual mice (6 mice/group) were stimulated with A20.2J cells loaded with a peptide encoding the PyCSP immunodominant CD8⁺ T cell epitope. Boolean gating was used to define the frequency of antigen-experienced CD8⁺ T cells (CD19⁻/NKp46⁻/CD3⁺/CD8⁺/CD44⁺ “bright”) expressing IFN-γ, IL-2 and/or TNF-α. The line within the box represents the mean and the bottom and top lines of the box represent the minimum and maximum responses observed in individual mice. **indicates statistical significance compared with the DNA-HuAd5 vaccine