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Table 2 Reduction of between-plate variation for MFI on the linear and natural-log scales

From: An analytical approach to reduce between-plate variation in multiplex assays that measure antibodies to Plasmodium falciparum antigens

Antigen Linear scale Natural-log scale
Unadjusted variance Adjusteda (I = 5) to unadjusted ratio Adjustedb (I = 6) to unadjusted ratio Adjustedc (I = 6) to unadjusted ratio Adjustedd (I = 7) to unadjusted ratio Unadjusted variance Adjusteda (I = 5) to unadjusted ratio Adjustedb (I = 6) to unadjusted ratio Adjustedc (I = 6) to unadjusted ratio Adjustedd (I = 7) to unadjusted ratio
Recombinant protein
 AMA-1 139,412 1.183 1.079 1.160 1.077 0.0013 1.024 0.912 1.026 0.925
 EBA175 125,497 1.013 0.966 1.269 1.151 0.0060 1.030 0.900 1.083 0.943
 MSP1 167,994 0.905 0.889 0.992 0.957 0.0121 0.609 0.598 0.598 0.606
 MSP2 759,821 0.928 0.927 0.873 0.863 0.0070 0.849 0.844 0.751 0.759
 MSP3 676,301 0.887 0.725 0.874 0.733 0.0076 0.801 0.658 0.903 0.631
 FV2 2,679,797 0.659 0.539 0.576 0.506 0.0739 0.473 0.445 0.452 0.444
Gene expression protein
 MSP11 37,028 0.842 0.875 0.811 0.850 0.0021 0.862 0.894 0.830 0.869
 Pf41 170,559 0.621 0.596 0.615 0.600 0.0155 0.279 0.289 0.288 0.299
Synthetic peptide
 CSP 157,499 0.742 0.760 0.727 0.743 0.0355 0.400 0.395 0.369 0.372
  1. The ratios assess the variance reduction of the different adjustments with values less than one indicating a reduction of between-plate compared to the unadjusted values.
  2. aAdjustment using 5 pooled controls from pre-screened samples (PC1–PC5)
  3. bAdjustment using 6 pooled controls including the pooled negative female control (PC1–PC5 plus US-NC)
  4. cAdjustment using 6 pooled controls including the pooled negative male control (PC1–PC5 plus FV2-NC)
  5. dAdjustment using all 7 pooled controls