Fig. 2
From: Generation of transgenic rodent malaria parasites by transfection of cell culture-derived merozoites
Experimental optimization of sporozoite numbers for HeLa cell infection to obtain maximal numbers of detached cells/merosomes. a, b HeLa cells were infected with different numbers of PbmCherryHsp70 parasites and counted at 48 hpi. Detached cells and merosomes were counted in the supernatant at 65 hpi. a Total number of detached cells formed at 65 hpi depending on the number of sporozoites used for infection. b Percentage of detached cells formed in relation to parasite count at 48 hpi was calculated. Shown are means with errors depicted as 95% confidence intervals, n = 3 in biological triplicates