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Table 2 shows the individual detached cell transfections performed that are summarized in Fig. 5a–c with information about the transfected construct, the parasite line that was transfected, the type of integration, the success rate, how often the transfection was performed and at which day after the transfection the mice became positive

From: Generation of transgenic rodent malaria parasites by transfection of cell culture-derived merozoites

Construct Parasite line Integration Success rate Number of transfections Day mice became positive
PbSBP1KO (P0B90-GFP) [19] mCherryHsp70 [20] Double crossover 50% (2) 2 7
PbSBP1KO (P0B90-GFP) [19] mCherryHsp70FLucef1a [21] Double crossover 100% (1) 2 6
PbSBP1KO (P0B90-GFP) [19] WT Double crossover 100% (1) 1 7
PfSBP1 (PL0017-mCherry) [19] PbSBP1KO [19] Single crossover 100% (1) 1 8
PbGEM-336027 mCherryHsp70 [20] Double crossover 100% (1) 1 21
PbNLuc (PL0017) [18] WT Single crossover 100% (1) 1 5
PbNLuc (PL0017) [18] mCherryHsp70 [20] Single crossover 100% (2) 2 4
PbNLuc (PL0017) [18] mCherryHsp70FLucef1a [21] Single crossover 66.6% (3) 3 4–5
PL0017 GFP [22] mCherryHsp70 [20] Single crossover 100% (8) 8 5–12