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Table 1 Details of the comparison of concentration methods. n/N = Infected mosquitoes/total number of mosquitoes dissected

From: Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays

Experiment

Protocol

Blood volume (µL)a

Total oocysts

Infected mosq. n/N (%)

p value*

Mean oocysts (range)

% change in mean oocyst to control

p value*

1

Control

300

18

9/20 (45%)

–

0.9 (0–5)

–

–

Centrifugation

1500

2

2/20 (10%)

0.031

0.1 (0–1)

−88.9

0.0112

Percoll

1500

18

11/20 (55%)

0.7524

0.9 (0–3)

0

0.6185

MACS

1500

85

17/20 (85%)

0.0187

4.3 (0–11)

377.8

0.0003

2

Control

300

230

18/20 (90%)

–

11.5 (0–27)

–

–

Centrifugation

1500

413

20/20 (100%)

0.4872

20.7 (2–46)

80

0.0011

Percoll

1500

225

18/20 (90%)

1

11.3 (0–23)

−1.7

0.8494

MACS

1500

482

20/20 (100%)

0.4872

24.1 (8–38)

109.6

<0.0001

3

Control

300

103

18/20 (80%)

–

5.2 (0–20)

–

–

Centrifugation

1500

0

0/20 (0%)

<0.0001

0 (0)

−100

<0.0001

Percoll

1500

313

19/20 (95%)

0.3416

15.7 (0–30)

201.9

0.0002

MACS

1500

135

20/20 (100%)

0.106

6.8 (1–14)

30.8 

0.2348

  1. * Significance compared to paired control
  2. aBefore concentration; blood volume in feeder 300 µL