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Table 1 Details of the comparison of concentration methods. n/N = Infected mosquitoes/total number of mosquitoes dissected

From: Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays

Experiment Protocol Blood volume (µL)a Total oocysts Infected mosq. n/N (%) p value* Mean oocysts (range) % change in mean oocyst to control p value*
1 Control 300 18 9/20 (45%) 0.9 (0–5)
Centrifugation 1500 2 2/20 (10%) 0.031 0.1 (0–1) −88.9 0.0112
Percoll 1500 18 11/20 (55%) 0.7524 0.9 (0–3) 0 0.6185
MACS 1500 85 17/20 (85%) 0.0187 4.3 (0–11) 377.8 0.0003
2 Control 300 230 18/20 (90%) 11.5 (0–27)
Centrifugation 1500 413 20/20 (100%) 0.4872 20.7 (2–46) 80 0.0011
Percoll 1500 225 18/20 (90%) 1 11.3 (0–23) −1.7 0.8494
MACS 1500 482 20/20 (100%) 0.4872 24.1 (8–38) 109.6 <0.0001
3 Control 300 103 18/20 (80%) 5.2 (0–20)
Centrifugation 1500 0 0/20 (0%) <0.0001 0 (0) −100 <0.0001
Percoll 1500 313 19/20 (95%) 0.3416 15.7 (0–30) 201.9 0.0002
MACS 1500 135 20/20 (100%) 0.106 6.8 (1–14) 30.8  0.2348
  1. * Significance compared to paired control
  2. aBefore concentration; blood volume in feeder 300 µL