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Table 1 Specific oligonucleotide primers used for PCR amplification

From: Prevalence of polymorphisms in glucose-6-phosphate dehydrogenase, sickle haemoglobin and nitric oxide synthase genes and their relationship with incidence of uncomplicated malaria in Iganga, Uganda

SNP (gene)

PCR round

PCR primers

Product size (bp)

Digesting enzyme

Fragment size (bp)

Digested (normal)

Digested (mutant)

rs 334 (HBB)

First

5′-AGAAAACATCAAGGGTCCCA-3′ (forward)

926

   

5′-TCCATCTACATATCCCAAAGC-3′ (reverse)

Nested

5′-TCCAAGGGTAGACCACCAGC-3′ (forward)

445

DdeI

225, 220

445

5′-GTGCCAGAAGAGCCAAGGAC-3′ (reverse)

rs 1050828 (G6PD)

Single

5′-GTGGCTGTTCCGGGATGGCCTTCTG-3′ (forward)

109

NlaII

109

63, 46

5′-CTTGAAGAAGGGCTCACTCTGTTTG-3′ (reverse)

rs 1800482 (NOS2)

Single

5′-CATATGTATGGGAATACTGTATTTCAGGC-3′

573

BsaI

446, 127

573

5′-TCTGAACTAGTCACTTGAGG-3′