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Fig. 4 | Malaria Journal

Fig. 4

From: A new method for sequencing the hypervariable Plasmodium falciparum gene var2csa from clinical samples

Fig. 4

Distribution of genetic diversity in the 5′ region of var2csa. a Distribution of nucleotide diversity and Tajima’s D across var2csa. Sliding window analyses of the nucleotide nucleotide diversity (blue) and Tajima’s D values (red) are plotted against the length of the coding region, with a window size of 99 nucleotides and step size of 30 nucleotides. The highest peak of nucleotide diversity corresponds to the inter domain 1 region. An asterisk denotes a position that significantly deviated from neutrality (p < 0.05), which included positions 666–764, 3563–3694, 3866–3964, and 4391–4573 in the alignment. The VAR2CSA segment analysed in this study is at the top of the plot. b Sliding window analysis of non-synonymous to synonymous mutation rate is plotted (π N/π S ratio) against the length of the coding region, with a window size of 99 nucleotides and step size of 30 nucleotides. The blue dashed line denotes a π N/π S ratio of 1; π N/π S >1 indicates an excess of non-synonymous mutations, whereas a ratio <1 is indicative of an excess of synonymous mutations. The VAR2CSA segment analysed in this study is shown above the plot

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