Table 1 Summary of in vitro gametocyte longevity data evaluated by three viability assays from cultures of one Plasmodium falciparum clinical isolate and two laboratory strains
From: Life-span of in vitro differentiated Plasmodium falciparum gametocytes
Plasmodium falciparum | Gametocyte longevity in days: mean (range) | |||
|---|---|---|---|---|
Microscopy | Flow cytometry | RT-qPCR | ||
Pfs25 | PF14_0367a | |||
3D7 | – | 46 (43–49) | – | – |
NF54 | 46 | 49 (48–50) | >52 | >52 |
JH013 | 39 (38–40) | 45 (43–49) | >52 | 46 |
Average number of days of gametocyte culture to reach peak gametocytaemia (maturation)c | 20.5 | 16 | 19 | 19 |
Mature gametocytes 50% survival rate (days) | 2.6 | 6.5 | 4.5 | 2.5 |
Mature gametocytes 10% survival rate (days) | 10 | 12 | 13.3 | 10.4 |
Mature gametocyte circulation time (days)b | 18–26 | 16–24 | 31–32 | 26–31 |