Skip to main content

Table 1 Summary of in vitro gametocyte longevity data evaluated by three viability assays from cultures of one Plasmodium falciparum clinical isolate and two laboratory strains

From: Life-span of in vitro differentiated Plasmodium falciparum gametocytes

Plasmodium falciparum Gametocyte longevity in days: mean (range)
Microscopy Flow cytometry RT-qPCR
Pfs25 PF14_0367a
3D7 46 (43–49)
NF54 46 49 (48–50) >52 >52
JH013 39 (38–40) 45 (43–49) >52 46
Average number of days of gametocyte culture to reach peak gametocytaemia (maturation)c 20.5 16 19 19
Mature gametocytes 50% survival rate (days) 2.6 6.5 4.5 2.5
Mature gametocytes 10% survival rate (days) 10 12 13.3 10.4
Mature gametocyte circulation time (days)b 18–26 16–24 31–32 26–31
  1. aMid and mature gametocyte marker [23]
  2. bCirculation time is calculated by subtracting the number of days needed to reach peak gametocytaemia
  3. cOn the day of peak gametocytaemia all gametocytes in the respective culture have reached maturity (stage V)