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Table 2 G6PD detection methods, phenotypes and genotypes

From: Safety of primaquine given to people with G6PD deficiency: systematic review of prospective studies

Study (country) Methods Results
Phenotype Genotype
Krudsood [24]
(Thailand)
Not reported G6PD normal: 134 patients
G6PD deficient: 7 patients
Not tested
Shekalaghe [22]
(Tanzania)
PCR for G202A, A376G G6PD normal: 77 patients
G6PD deficient: 30 patients
G6PD wild type: 77 patients
G6PD deficient heterozygous: 23 patients
G6PD deficient hemizygous/homozygous: 7 patients
Shekalaghe [23]
(Tanzania)
PCR for G202A, A376G G6PD normal: 493 patients
G6PD deficient: 69 patients
G6PD wild type: 493 patients
G6PD deficient heterozygous: 47 patients
G6PD deficient hemizygous/homozygous: 22 patients
Rueangweerayut [32]
(Thailand)
PCR RFLP for Mahidol G6PD normal (Mahidol negative with >80% G6PD activity): 6 patients
G6PD deficient (G6PD enzyme activity 40–60% of normal): 6 patients
G6PD wild type: 6 patients
G6PD deficient heterozygous: 6 patients
Eziefula [27, 33]a
(Uganda)
FST; PCR for G202A/A376G G6PD normal: 373 patients
G6PD deficient: 88 patients
G6PD wild type: 373 patients
G6PD deficient heterozygous: 61 patients
G6PD deficient hemizygous/homozygous: 27 patients
Kheng [30]
(Cambodia)
FST; enzyme quantification; PCR for Mahidol, Mediterranean, Coimbra, Chinese-5, and Canton G6PD normal: 57 patients
G6PD deficient: 18 patients
G6PD wild type: 57 patients
G6PD deficient hemizygous male: 14 patients
G6PD deficient homozygous female: 1 patient
G6PD deficient heterozygous female: 3 patients
Bancone [29]
(Thai–Myanmar border)
FST; enzyme quantification; PCR RFLP for Mahidol. Chinese-4, Canton and Viangchan G6PD normal: 1226 patients
G6PD intermediate: 50 patients
G6PD deficient: 124 patients
G6PD wild type: 1226 patients
G6PD deficient hemizygous male: 39 patients
G6PD deficient homozygous female: 5 patient
G6PD deficient heterozygous female: 65 patients
Ley [31]
(Bangladesh)
FST; enzyme quantification G6PD normal: 169
Mild G6PD deficiency: 5 patients
Severe G6PD deficiency: 1 patient
Not tested
Mwaiswelo [28]
(Tanzania)
Rapid test; PCR for G202A, A376G G6PD normal: 184 patients
G6PD deficient: 33 patients
Males
G6PD A wild type: 95 patients
G6PD A− hemizygous: 15 patients
Females
G6PD AA and BA wild type: 83 patients
G6PD A−A− homozygous: 5 patients
G6PD AA− and BA−heterozygous: 22 patients
  1. FST fluorescent spot test, PCR polymerase chain reaction, RFLP restriction fragment length polymorphism (RFLP)
  2. aOnly G6PD ‘normal’ participants based on the FST test; those not normal G6PD by FST were excluded and the genotyping was done on the remainder