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Table 3 Microsatellite markers used in this study, repeated pattern (RP) from Zheng et al. [50] and characteristics (allele size, AS; annealing temperature, Ta)

From: The implementation of long-lasting insecticidal bed nets has differential effects on the genetic structure of the African malaria vectors in the Anopheles gambiae complex in Dielmo, Senegal

Marker locus

RP

Sequence primer flanking

AS (bp)

Ta (°C)

An. coluzzii (N = 119)

An. gambiae (N = 82)

An. arabiensis (N = 309)

Na

A

Na

A

Na

A

H59

(GT)9

5′ CCCCTATTAAACCCTGGACG 3′

3′ TGTTGTTGCCCTGCGTTACC 5′

123

55

10

6.75 ± 1.71

7

6.00 ± 1.00

13

7.33 ± 1.50

H88

(GT)9

5′TGCGGCGGTAAAGCATCAAC 3′

3′ CCGGTAACACTGCGCCGAC 5′

176

60

NPA

NPA

NPA

NPA

23

13.44 ± 3.05

H93

(GT)4 + 7

5′ TCCCCAGCTCACCCTTCAAG 3′

3′ GGTTGCATGTTTGGATAGCG 5′

209

55

19

10.75 ± 3.86

20

12.67 ± 0.58

30

13.00 ± 3.81

H119

(GT)6

5′ GGTTGATGCTGAAGAGTGGG 3′

3′ ATGCCAGCGGATACGATTCG 5′

174

55

17

12.25 ± 1.26

14

10.67 ± 0.58

10

6.89 ± 1.69

H128

(GT)21

5′ CGGGACGGCTAGATAAAGCG 3′

3′ CCGGGCGACATAACCCACCC 5′

117

55

25

17.5 ± 3.42

24

16.67 ± 3.22

b

b

H242

(GT)8

5′TTCATTTCCACCGCAGCTGC 3′

3′GGCGACACTCAATCCTTCC 5′

69

58

7

5.50 ± 1.00

7

5.67 ± 0.58

c

c

H249

(GT)15

5′ ATGTTCCGCACTTCCGACAC 3′

3′ GCGAGCTACAACAATGGAGC 5′

129

60

22

13.50 ± 1.73

17

12.33 ± 1.16

27

12.67 ± 2.69

H312

(GT)10

5′ TAAACCATCAACCAGCCACC 3′

3′ ACTGTGTGCGAGTCGGTTGG 5′

108

58

19

12.75 ± 1.50

16

13.00 ± 0.00

29

15.33 ± 4.79

H555

(GT)8

5′GCAGAGACACTTTCCGAAAC3′

3′ TGTCAACCCACATTTTGCGC 5′

81

55

17

10.50 ± 3.00

15

9.67 ± 3.06

b

b

H577

(GT)16

5′ TTCAGCTTCAGGTTGGTCTC 3′

3′ GGGTTTTTTGGCTGCGACTG 5′

113

55

13

8.50 ± 2.65

14

10.00 ± 1.00

22

10.78 ± 3.31

H746

(GT)14

5′ TGGGTTCGAAATTCGCCAAC 3′

3′ GACGTGTGCACCCGTTGTG 5′

105

58

14

11.25 ± 1.26

13

10.67 ± 1.16

29

16.89 ± 2.57

H758

(GT)11

5′ TGATTTGCCAGTTCTGCCAG 3′

3′ GTGATTGGAGTGGCTAGTGG 5′

105

60

a

a

a

a

11

5.11 ± 0.93

H812

(GT)10

5′CTGGCCCATTTTGCATATGC 3′

3′ TGCTCCACCAAACCACATC 5′

131

55

a

a

a

a

15

10.00 ± 1.58

H817

(GT)8

5′ ACTGGTCCGTTGCTGCGCG 3′

3′ ATGAGTGAATGGTGCGCTGG 5′

124

58

7

6.00 ± 0.82

6

5.00 ± 1.00

b

b

Mean

    

15.46 ± 5.87

10.48 ± 3.89

13.91 ± 5.59

10.21 ± 3.76

20.90 ± 7.56

11.14 ± 4.14

  1. Parameters of polymorphism for An. gambiae, An. coluzzii and An. arabiensis populations are given: number of observed alleles (Na), mean number of alleles per locus (A), number of specimens used (N)
  2. aNull alleles in An. gambiae and An. coluzzii; b null alleles in An. arabiensis, c monomorphic locus, not used in the study, NPA not properly amplified