Fig. 1

Ned-19 specific blockage of P. falciparum asexual growth. a Structure of Ned-19 and of its inactive analogue Ned-20, adapted from Rosen et al. b Synchronous early ring stage parasites (initial parasitaemia 0.16%) were cultured for 48 h in the presence of 100 μM concentration of the indicated compounds. At the end of the incubation, parasitaemias were measured through Giemsa-stained preparations. Parasitaemia of the DMSO treated culture (1.08%) was set as 1. N = 3. ***p < 0.001. Error bar SEM. c Late asexual parasites (24 h post invasion, initial parasitaemia 1%) were cultured with the indicated concentrations of Ned-19 for 24 h. Parasitaemia was then measured through FACS counting of at least 50.000 cells per sample. N = 2. *p < 0.05; ***p < 0.001; ****p < 0.0001. Error bar range/2