Fig. 4

Controlling for contamination and false positives. a Wiping scissors and forceps three times with a kimwipe sprayed with 70% ethanol is sufficient to prevent contamination when cutting from a high to a zero parasitemic sample. b The amplification curves for all of the 0 parasites/mL samples are shown indicating lack of amplification. c Representative plates from a field survey from Myanmar showing that even in high malaria burden areas, negative control samples consisting of blank filter papers (indicated by asterisk) remain negative