Table 1 Limits of detection (LoD) for home-made and commercial new extraction method (NEM) buffers using Whatman 3MM dried blood spots
Step | Home-made | Commercial |
|---|---|---|
Lysis | 3M Guanidine thiocyanate 16.7% Isopropanol 2% Triton X100 10 mM EDTA 5 mM Trizma HCl pH 7.4 0.1% 6 N HCl 0.5% 2-mercaptoethanol pH 6.0–6.5 | Qiagen RLT-plus 16.7% Isopropanol 0.5% 2-mercaptoethanol |
Wash 1 | Same as lysis but no 2-mercaptoethanol | Same as lysis but no 2-mercaptoethanol |
Wash 2 | 25% Ethanol 25% Isopropanol 100 mM Sodium chloride 10 mM Trizma HCl pH 7.4 | 70% Ethanol 30% PBS |
LoD | 20 parasites/mL | 22 parasites/mL |