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Table 1 Limits of detection (LoD) for home-made and commercial new extraction method (NEM) buffers using Whatman 3MM dried blood spots

From: A novel method for extracting nucleic acids from dried blood spots for ultrasensitive detection of low-density Plasmodium falciparum and Plasmodium vivax infections

Step Home-made Commercial
Lysis 3M Guanidine thiocyanate 16.7% Isopropanol 2% Triton X100 10 mM EDTA 5 mM Trizma HCl pH 7.4 0.1% 6 N HCl 0.5% 2-mercaptoethanol pH 6.0–6.5 Qiagen RLT-plus 16.7% Isopropanol 0.5% 2-mercaptoethanol
Wash 1 Same as lysis but no 2-mercaptoethanol Same as lysis but no 2-mercaptoethanol
Wash 2 25% Ethanol 25% Isopropanol 100 mM Sodium chloride 10 mM Trizma HCl pH 7.4 70% Ethanol 30% PBS
LoD 20 parasites/mL 22 parasites/mL