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Table 1 Limits of detection (LoD) for home-made and commercial new extraction method (NEM) buffers using Whatman 3MM dried blood spots

From: A novel method for extracting nucleic acids from dried blood spots for ultrasensitive detection of low-density Plasmodium falciparum and Plasmodium vivax infections

Step Home-made Commercial
Lysis 3M Guanidine thiocyanate
16.7% Isopropanol
2% Triton X100
10 mM EDTA
5 mM Trizma HCl pH 7.4
0.1% 6 N HCl
0.5% 2-mercaptoethanol
pH 6.0–6.5
Qiagen RLT-plus
16.7% Isopropanol
0.5% 2-mercaptoethanol
Wash 1 Same as lysis but no
2-mercaptoethanol
Same as lysis but no
2-mercaptoethanol
Wash 2 25% Ethanol
25% Isopropanol
100 mM Sodium chloride
10 mM Trizma HCl pH 7.4
70% Ethanol
30% PBS
LoD 20 parasites/mL 22 parasites/mL