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Table 2 Nested PCR assays using Plasmodium genus and species-specific primers

From: Absence of Plasmodium inui and Plasmodium cynomolgi, but detection of Plasmodium knowlesi and Plasmodium vivax infections in asymptomatic humans in the Betong division of Sarawak, Malaysian Borneo

Sample trip number Number samples tested Pool 2a samples Pool 1b samples Genus positive onlyc Speciesd
Pk Pf Pm Po Pv Pin Pcy
1 256 5 3 0 0 0 0 0 0 0 0
2 349 3 3 0 3 0 0 0 0 0 0
3 326 4 4 0 2 0 0 0 1 0 0
4 304 5 1 0 0 0 0 0 0 0 0
5 338 3 5 0 2 0 0 0 0 0 0
6 288 0 0 0 0 0 0 0 0 0 0
7 257 0 0 0 0 0 0 0 0 0 0
8 257 ND ND 1 0 0 0 0 0 0 0
9 254 ND ND 0 0 0 0 0 0 0 0
10 257 ND ND 0 0 0 0 0 0 0 0
11 116 ND ND 0 0 0 0 0 0 0 0
Total 3002 66 86 1 7 0 0 0 1 0 0
  1. ND not done
  2. aPool 2 samples contained DNA extracted from 4 persons
  3. bPool 1 samples contained DNA extracted from 2 persons
  4. cThe number of individually extracted DNA samples which were positive for Plasmodium genus-specific primers but negative for all of the Plasmodium species-specific primers
  5. dThe number of samples which were positive for Plasmodium species-specific primers: P. knowlesi: Pk; P. falciparum: Pf; P. malariae: Pm; P. ovale: Po; P. vivax: Pv; P. inui: Pin; P. cynomolgi: Pcy
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