Absence of Plasmodium inui and Plasmodium cynomolgi, but detection of Plasmodium knowlesi and Plasmodium vivax infections in asymptomatic humans in the Betong division of Sarawak, Malaysian Borneo

Table 2 Nested PCR assays using Plasmodium genus and species-specific primers

Sample trip number	Number samples tested	Pool 2^a samples	Pool 1^b samples	Genus positive only^c	Species^d
Sample trip number	Number samples tested	Pool 2^a samples	Pool 1^b samples	Genus positive only^c	Pk	Pf	Pm	Po	Pv	Pin	Pcy
1	256	5	3	0	0	0	0	0	0	0	0
2	349	3	3	0	3	0	0	0	0	0	0
3	326	4	4	0	2	0	0	0	1	0	0
4	304	5	1	0	0	0	0	0	0	0	0
5	338	3	5	0	2	0	0	0	0	0	0
6	288	0	0	0	0	0	0	0	0	0	0
7	257	0	0	0	0	0	0	0	0	0	0
8	257	ND	ND	1	0	0	0	0	0	0	0
9	254	ND	ND	0	0	0	0	0	0	0	0
10	257	ND	ND	0	0	0	0	0	0	0	0
11	116	ND	ND	0	0	0	0	0	0	0	0
Total	3002	66	86	1	7	0	0	0	1	0	0

ND not done
^aPool 2 samples contained DNA extracted from 4 persons
^bPool 1 samples contained DNA extracted from 2 persons
^cThe number of individually extracted DNA samples which were positive for Plasmodium genus-specific primers but negative for all of the Plasmodium species-specific primers
^dThe number of samples which were positive for Plasmodium species-specific primers: P. knowlesi: Pk; P. falciparum: Pf; P. malariae: Pm; P. ovale: Po; P. vivax: Pv; P. inui: Pin; P. cynomolgi: Pcy

ISSN: 1475-2875