Sample

Illumigene

RDT

Microscopy

PCR

Parasite density (parasites/µL)


Result

Species


1

Pos

Pos

Pos

Pos

Pf + Po

1,027,798

2

Pos

Pos

Pos

Pos

Pf

292,432

3

Pos

Pos

Pos

Pos

Pv

68,129

4

Pos

Pos

Pos

Pos

Pf

27,731

5

Pos

Pos

Pos

Pos

Pv

7285

6

Pos

Pos

Pos

Pos

Pf

5917

7

Pos

Pos

Pos

Pos

Pf + Po

98

8

Pos

Pos (*)

Pos

Pos

Po

1496

9

Pos

Neg

Pos

Pos

Po

59

10

Pos

Neg

Pos

Pos

Pv

340

11

Pos

Neg

Pos

Pos

Pf

415

12

Pos

Pos

Neg

− (#)

–

–

13

Neg

Neg

Neg

/
  
14

Neg

Neg

Neg

/
  
15

Neg

Neg

Neg

/
  
16

Neg

Neg

Neg

/
  
17

Neg

Neg

Neg

/
  
18

Neg

Neg

Neg

/
  
19

Neg

Neg

Neg

/
  
20

Neg

Neg

Neg

/
  
21

Neg

Neg

Neg

/
  
22

Neg

Neg

Neg

/
  
23

Neg

Neg

Neg

/
  
24

Neg

Neg

Neg

/
  
25

Neg

Neg

Neg

/
  
26

Neg

Neg

Neg

/
  
27

Neg

Neg

Neg

/
  
28

Neg

Neg

Neg

/
  
29

Neg

Neg

Neg

/
  
30

Neg

Neg

Neg

/
  
 Parasite density as quantified by microscopy on blood smear
 RDT, rapid diagnostic test; PCR, fourprimer realtime PCR; Pf, Plasmodium falciparum; Pm, Plasmodium malariae; Po, Plasmodium ovale; Pv, Plasmodium vivax; Pos, positive; Neg, negative. –, missing data; (#), negative result with realtime PCR performed on thin blood smear, possibly due to inferior sample quality; (*), difficult to interpret (weak positive result); /, triple negative results (i.e. negative with RDT, microscopy and illumigene malaria assay) were not sent to the ITM for confirmation with realtime PCR