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Table 5 Comparison of compound EC50 values obtained by fluorescent (SYBR Green), and radiometric ([3H]-hypoxanthine) assay platforms

From: Screening a protein kinase inhibitor library against Plasmodium falciparum

MMV series

Compound

EC50, mM

Ratio fluorescent vs radiometric

Fluorescent

C.I. 95%, n = 4

Radiometric

C.I. 95%, n = 2

MMV02

A0002

0.23 ± 0.04

0.06 ± 0.07

4

MMV03

A0003

0.59 ± 0.12

0.15 ± 0.25

4

MMV04

A0004

0.70 ± 0.07

0.41 ± 0.47

2

MMV05

A0005

0.01 ± 0.00

0.01 ± 0.00

1

MMV06

A0006

0.04 ± 0.01

0.04 ± 0.01

1

MMV08

A0008

0.56 ± 0.09

0.30 ± 0.10

2

MMV09

A0009

0.23 ± 0.09

0.15 ± 0.09

2

MMV10

A0010

0.55 ± 0.08

0.35 ± 0.13

2

MMV11

A0011

0.64 ± 0.10

0.19 ± 0.02

3