Table 5 Comparison of compound EC50 values obtained by fluorescent (SYBR Green), and radiometric ([3H]-hypoxanthine) assay platforms
From: Screening a protein kinase inhibitor library against Plasmodium falciparum
MMV series | Compound | EC50, mM | Ratio fluorescent vs radiometric | |
|---|---|---|---|---|
Fluorescent C.I. 95%, n = 4 | Radiometric C.I. 95%, n = 2 | |||
MMV02 | A0002 | 0.23 ± 0.04 | 0.06 ± 0.07 | 4 |
MMV03 | A0003 | 0.59 ± 0.12 | 0.15 ± 0.25 | 4 |
MMV04 | A0004 | 0.70 ± 0.07 | 0.41 ± 0.47 | 2 |
MMV05 | A0005 | 0.01 ± 0.00 | 0.01 ± 0.00 | 1 |
MMV06 | A0006 | 0.04 ± 0.01 | 0.04 ± 0.01 | 1 |
MMV08 | A0008 | 0.56 ± 0.09 | 0.30 ± 0.10 | 2 |
MMV09 | A0009 | 0.23 ± 0.09 | 0.15 ± 0.09 | 2 |
MMV10 | A0010 | 0.55 ± 0.08 | 0.35 ± 0.13 | 2 |
MMV11 | A0011 | 0.64 ± 0.10 | 0.19 ± 0.02 | 3 |