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Table 5 Comparison of compound EC50 values obtained by fluorescent (SYBR Green), and radiometric ([3H]-hypoxanthine) assay platforms

From: Screening a protein kinase inhibitor library against Plasmodium falciparum

MMV series Compound EC50, mM Ratio fluorescent vs radiometric
Fluorescent
C.I. 95%, n = 4
Radiometric
C.I. 95%, n = 2
MMV02 A0002 0.23 ± 0.04 0.06 ± 0.07 4
MMV03 A0003 0.59 ± 0.12 0.15 ± 0.25 4
MMV04 A0004 0.70 ± 0.07 0.41 ± 0.47 2
MMV05 A0005 0.01 ± 0.00 0.01 ± 0.00 1
MMV06 A0006 0.04 ± 0.01 0.04 ± 0.01 1
MMV08 A0008 0.56 ± 0.09 0.30 ± 0.10 2
MMV09 A0009 0.23 ± 0.09 0.15 ± 0.09 2
MMV10 A0010 0.55 ± 0.08 0.35 ± 0.13 2
MMV11 A0011 0.64 ± 0.10 0.19 ± 0.02 3