Fig. 3

Rapamycin alters splenic lymphocyte migratory capacity and effector functions. a Ex vivo luciferase activity in spleen on day 6 after infection of mice with transgenic luciferase-expressing P. berghei ANKA treated with vehicle or rapamycin (5 mg/kg) on day 4. Total numbers of splenocytes, spleen lymphocytes and relative numbers of CD4+ and CD8+ T cells of vehicle (V) or rapamycin treated (R) mice on day 6 post infection. b Relative CD8+ lymphocyte numbers, with representative dot plots of activated CD8+ T cell populations expressing the indicated markers in the spleen of the indicated treatment groups on day 6 post infection. c Relative numbers with representative dot plots of granzyme B+ effector T cells gated from the CD3+ CD69+ LFA-1+ population. d Relative numbers of CD62L+ naïve CD8+ T cells gated from CD3+ splenocytes with representative dot plots from the indicated treatment group on day 6 post infection. Data are mean ± SEM; n = 5/group