Fig. 2

In vivo imaging using the microvascular microscope. a The microvascular microscope (MvM) was used for in vivo investigations to detect haemozoin circulating in the superficial microvasculature. The MvM employs cross polarized epi-illumination to optically access vessels below the tissue surface. LED illumination is collimated by an achromatic collimating lens (CL), polarized using a linear polarizer (LP) followed by a collinear polarizing beam splitter (PBS), and finally delivered to the tissue through a microscope objective. Diffusely scattered depolarized light is collected by the microscope objective and imaged onto a CCD detector. An example field of view from an uninfected subject is shown in the bottom row. Vessels were located under green light illumination (b) and red light illumination was used to detect haemozoin circulating through the vessel (c)